טוען...
High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR/Cas9 in yeast
Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (deletions, substitutions, and insertions) with an e...
שמור ב:
| הוצא לאור ב: | Nat Biotechnol |
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| Main Authors: | , , , , , , , , , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
2018
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5990468/ https://ncbi.nlm.nih.gov/pubmed/29786095 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4147 |
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