High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR/Cas9 in yeast

Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (deletions, substitutions, and insertions) with an e...

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Veröffentlicht in:Nat Biotechnol
Hauptverfasser: Guo, Xiaoge, Chavez, Alejandro, Tung, Angela, Chan, Yingleong, Kaas, Christian, Yin, Yi, Cecchi, Ryan, Garnier, Santiago Lopez, Kelsic, Eric, Schubert, Max, DiCarlo, James E., Collins, James J., Church, George M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5990468/
https://ncbi.nlm.nih.gov/pubmed/29786095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4147
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