Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

A protocol is presented for generating human induced pluripotent stem cells (hiPSCs) that express endogenous proteins fused to in-frame N- or C-terminal fluorescent tags. The prokaryotic CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) may be used to...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Autores principales: Haupt, Amanda, Grancharova, Tanya, Arakaki, Joy, Fuqua, Margaret A., Roberts, Brock, Gunawardane, Ruwanthi N.
Formato: Artigo
Lenguaje:Inglês
Publicado: MyJove Corporation 2018
Materias:
Genetics
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6231893/
https://ncbi.nlm.nih.gov/pubmed/30199041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58130
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