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Evaluation and optimisation of unnatural amino acid incorporation and bioorthogonal bioconjugation for site-specific fluorescent labelling of proteins expressed in mammalian cells

Many biophysical techniques that are available to study the structure, function and dynamics of cellular constituents require modification of the target molecules. Site-specific labelling of a protein is of particular interest for fluorescence-based single-molecule measurements including single-mole...

詳細記述

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書誌詳細
出版年:Biochem Biophys Rep
主要な著者: Jakob, Leonhard, Gust, Alexander, Grohmann, Dina
フォーマット: Artigo
言語:Inglês
出版事項: Elsevier 2018
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC6226565/
https://ncbi.nlm.nih.gov/pubmed/30450427
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2018.10.011
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