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Genetic Incorporation of the Unnatural Amino Acid p-Acetyl Phenylalanine into Proteins for Site-Directed Spin Labeling

Site-directed spin labeling (SDSL) is a powerful tool for the characterization of protein structure and dynamics; however, its application in many systems is hampered by the reliance on unique and benign cysteine substitutions for the site-specific attachment of the spin label. An elegant solution t...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Evans, Eric G.B., Millhauser, Glenn L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4841275/
https://ncbi.nlm.nih.gov/pubmed/26478497
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.06.008
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