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Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling

Amplification and sequencing of 16S amplicons are widely used for profiling the structure of oral microbiota. However, it remains not clear whether and to what degree DNA extraction and targeted 16S rRNA hypervariable regions influence the analysis. Based on a mock community consisting of five oral...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Sci Rep
Main Authors: Teng, Fei, Darveekaran Nair, Sree Sankar, Zhu, Pengfei, Li, Shanshan, Huang, Shi, Li, Xiaolan, Xu, Jian, Yang, Fang
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6218491/
https://ncbi.nlm.nih.gov/pubmed/30397210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-34294-x
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