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Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling
Amplification and sequencing of 16S amplicons are widely used for profiling the structure of oral microbiota. However, it remains not clear whether and to what degree DNA extraction and targeted 16S rRNA hypervariable regions influence the analysis. Based on a mock community consisting of five oral...
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| Vydáno v: | Sci Rep |
|---|---|
| Hlavní autoři: | , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Nature Publishing Group UK
2018
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6218491/ https://ncbi.nlm.nih.gov/pubmed/30397210 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-34294-x |
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