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Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing

BACKGROUND AND OBJECTIVE: The advent of next-generation sequencing has significantly facilitated characterization of the oral microbiome. Despite great efforts in streamlining the processes of sequencing and data curation, upstream steps required for amplicon library generation could still influence...

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Detalhes bibliográficos
Main Authors: Abusleme, Loreto, Hong, Bo-Young, Dupuy, Amanda K., Strausbaugh, Linda D., Diaz, Patricia I.
Formato: Artigo
Idioma:Inglês
Publicado em: Co-Action Publishing 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4000428/
https://ncbi.nlm.nih.gov/pubmed/24778776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3402/jom.v6.23990
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