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How to Process Sputum Samples and Extract Bacterial DNA for Microbiota Analysis
Different steps and conditions for DNA extraction for microbiota analysis in sputum have been reported in the literature. We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA extraction technique for the microbiota analysis of...
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| Publicado no: | Int J Mol Sci |
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| Main Authors: | , , , , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MDPI
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6214103/ https://ncbi.nlm.nih.gov/pubmed/30347804 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms19103256 |
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