How to Process Sputum Samples and Extract Bacterial DNA for Microbiota Analysis

Different steps and conditions for DNA extraction for microbiota analysis in sputum have been reported in the literature. We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA extraction technique for the microbiota analysis of...

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Vydáno v:Int J Mol Sci
Hlavní autoři: Terranova, Leonardo, Oriano, Martina, Teri, Antonio, Ruggiero, Luca, Tafuro, Camilla, Marchisio, Paola, Gramegna, Andrea, Contarini, Martina, Franceschi, Elisa, Sottotetti, Samantha, Cariani, Lisa, Bevivino, Annamaria, Chalmers, James D., Aliberti, Stefano, Blasi, Francesco
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2018
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6214103/
https://ncbi.nlm.nih.gov/pubmed/30347804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms19103256
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