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Genetic Engineering of Human Pluripotent Stem Cells using PiggyBac Transposon System: hPSC engineering with PiggyBac transposons

Human pluripotent stem cells (hPSCs) emerged as an important tool to investigate human development and diseases. These studies often require genetically engineering hPSCs to stably express a transgene, which remains functional in various hPSC progeny. PiggyBac transposon is a highly effective and te...

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Veröffentlicht in:Curr Protoc Stem Cell Biol
Hauptverfasser: Ae Park, Mi, Sun Jung, Ho, Slukvin, Igor
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6212322/
https://ncbi.nlm.nih.gov/pubmed/30281932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpsc.63
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