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Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon

Induced pluripotent stem cells (iPSCs) have been generated from somatic cells by transgenic expression of Oct4, Sox2, Klf4, and cMyc. A major difficulty in the application of this technology for regenerative medicine, however, is the delivery of reprogramming factors. Whereas retroviral transduction...

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Main Authors: Yusa, Kosuke, Rad, Roland, Takeda, Junji, Bradley, Allan
格式: Artigo
語言:Inglês
出版: 2009
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2677165/
https://ncbi.nlm.nih.gov/pubmed/19337237
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1323
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