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A Versatile Lentiviral Delivery Toolkit for Proximity-dependent Biotinylation in Diverse Cell Types
Proximity-dependent biotinylation strategies have emerged as powerful tools to characterize the subcellular context of proteins in living cells. The popular BioID approach employs an abortive E. coli biotin ligase mutant (R118G; denoted as BirA*), which when fused to a bait protein enables the coval...
में बचाया:
| में प्रकाशित: | Mol Cell Proteomics |
|---|---|
| मुख्य लेखकों: | , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
The American Society for Biochemistry and Molecular Biology
2018
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6210231/ https://ncbi.nlm.nih.gov/pubmed/29991506 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR118.000902 |
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