A Versatile Lentiviral Delivery Toolkit for Proximity-dependent Biotinylation in Diverse Cell Types

Proximity-dependent biotinylation strategies have emerged as powerful tools to characterize the subcellular context of proteins in living cells. The popular BioID approach employs an abortive E. coli biotin ligase mutant (R118G; denoted as BirA*), which when fused to a bait protein enables the coval...

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Bibliographic Details
Published in:Mol Cell Proteomics
Main Authors: Samavarchi-Tehrani, Payman, Abdouni, Hala, Samson, Reuben, Gingras, Anne-Claude
Format: Artigo
Language:Inglês
Published: The American Society for Biochemistry and Molecular Biology 2018
Subjects:
Technological Innovation and Resources
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6210231/
https://ncbi.nlm.nih.gov/pubmed/29991506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR118.000902
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