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A Versatile Lentiviral Delivery Toolkit for Proximity-dependent Biotinylation in Diverse Cell Types
Proximity-dependent biotinylation strategies have emerged as powerful tools to characterize the subcellular context of proteins in living cells. The popular BioID approach employs an abortive E. coli biotin ligase mutant (R118G; denoted as BirA*), which when fused to a bait protein enables the coval...
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| Опубликовано в: : | Mol Cell Proteomics |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
The American Society for Biochemistry and Molecular Biology
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6210231/ https://ncbi.nlm.nih.gov/pubmed/29991506 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR118.000902 |
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