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Analyzing the Function of the Insert Region Found Between the α and β-Subunits in the Eukaryotic Nitrile Hydratase from Monosiga brevicollis

The functional roles of the (His)(17) region and an insert region in the eukaryotic nitrile hydratase (NHase, EC 4.2.1.84) from Monosiga brevicollis (MbNHase), were examined. Two deletion mutants, MbNHase(Δ238−257) and MbNHase(Δ219−272), were prepared in which the (His)(17) sequence and the entire i...

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Détails bibliographiques
Publié dans:Arch Biochem Biophys
Auteurs principaux: Yang, Xinhang, Bennett, Brian, Holz, Richard C.
Format: Artigo
Langue:Inglês
Publié: 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6201762/
https://ncbi.nlm.nih.gov/pubmed/30205086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2018.08.013
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