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Analyzing the Function of the Insert Region Found Between the α and β-Subunits in the Eukaryotic Nitrile Hydratase from Monosiga brevicollis

The functional roles of the (His)(17) region and an insert region in the eukaryotic nitrile hydratase (NHase, EC 4.2.1.84) from Monosiga brevicollis (MbNHase), were examined. Two deletion mutants, MbNHase(Δ238−257) and MbNHase(Δ219−272), were prepared in which the (His)(17) sequence and the entire i...

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Bibliographische Detailangaben
Veröffentlicht in:Arch Biochem Biophys
Hauptverfasser: Yang, Xinhang, Bennett, Brian, Holz, Richard C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6201762/
https://ncbi.nlm.nih.gov/pubmed/30205086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2018.08.013
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