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Analyzing the Function of the Insert Region Found Between the α and β-Subunits in the Eukaryotic Nitrile Hydratase from Monosiga brevicollis

The functional roles of the (His)(17) region and an insert region in the eukaryotic nitrile hydratase (NHase, EC 4.2.1.84) from Monosiga brevicollis (MbNHase), were examined. Two deletion mutants, MbNHase(Δ238−257) and MbNHase(Δ219−272), were prepared in which the (His)(17) sequence and the entire i...

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Detalhes bibliográficos
Publicado no:Arch Biochem Biophys
Main Authors: Yang, Xinhang, Bennett, Brian, Holz, Richard C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6201762/
https://ncbi.nlm.nih.gov/pubmed/30205086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2018.08.013
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