An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae

Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to unders...

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Bibliographic Details
Published in:MethodsX
Main Authors: Bresciani, Erica, Broadbridge, Elizabeth, Liu, Paul P.
Format: Artigo
Language:Inglês
Published: Elsevier 2018
Subjects:
Biochemistry, Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6197777/
https://ncbi.nlm.nih.gov/pubmed/30364607
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2018.10.009
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