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Protocol for Dissection and Dissociation of Zebrafish Telencephalon for Single-Cell Sequencing

Single-cell sequencing (sc-Seq) is a powerful tool to investigate the molecular signatures of cell types in a complex mixture of cells. A critical step in sc-Seq is preparing a single-cell suspension with a high number of viable cells. Here, we show how to dissect zebrafish telencephalon and how to...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Cosacak, Mehmet Ilyas, Bhattarai, Prabesh, Kizil, Caghan
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7263268/
https://ncbi.nlm.nih.gov/pubmed/32835293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100042
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