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Using a phiC31 “Disintegrase” to make new attP sites in the Drosophila genome at locations showing chromosomal position effects

An engineered phiC31 “Disintegrase” able to make an attP site in Drosophila out of an attR-attL pair is described. This was used to generate attP sites at genomic locations where a mini-white (mini-w) transgene was subject to chromosomal position effects (CPE). The first step was random genomic inte...

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Vydáno v:PLoS One
Hlavní autoři: Maharjan, Mukesh, Maeda, Robert K., Karch, François, Hart, Craig M.
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6175522/
https://ncbi.nlm.nih.gov/pubmed/30296303
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0205538
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