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Robust ΦC31-Mediated Genome Engineering in Drosophila melanogaster Using Minimal attP/attB Phage Sites
Effective genome engineering should lead to a desired locus change with minimal adverse impact to the genome itself. However, flanking loci with site-directed recombinase recognition sites, such as those of the phage ΦC31 integrase, allows for creation of platforms for cassette exchange and manipula...
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| Published in: | G3 (Bethesda) |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Genetics Society of America
2018
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5940133/ https://ncbi.nlm.nih.gov/pubmed/29523637 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.118.200051 |
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