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Robust ΦC31-Mediated Genome Engineering in Drosophila melanogaster Using Minimal attP/attB Phage Sites

Effective genome engineering should lead to a desired locus change with minimal adverse impact to the genome itself. However, flanking loci with site-directed recombinase recognition sites, such as those of the phage ΦC31 integrase, allows for creation of platforms for cassette exchange and manipula...

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Bibliografske podrobnosti
izdano v:G3 (Bethesda)
Main Authors: Voutev, Roumen, Mann, Richard S.
Format: Artigo
Jezik:Inglês
Izdano: Genetics Society of America 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5940133/
https://ncbi.nlm.nih.gov/pubmed/29523637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.118.200051
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