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Top‐down and bottom‐up characterization of nitrated birch pollen allergen Bet v 1a with CZE hyphenated to an Orbitrap mass spectrometer

Tyrosine (Tyr) residues of the major pollen allergen of birch Betula verrucosa, Bet v 1a, were nitrated by peroxynitrite. This modification enhances the allergenicity. Modified tyrosines were identified by analyzing intact allergen variants in combination with top‐down and bottom‐up approaches. Ther...

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Bibliographische Detailangaben
Veröffentlicht in:Electrophoresis
Hauptverfasser: Gusenkov, Sergey, Stutz, Hanno
Format: Artigo
Sprache:Inglês
Veröffentlicht: John Wiley and Sons Inc. 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6175448/
https://ncbi.nlm.nih.gov/pubmed/29389018
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201700413
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