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Construction of a new T-vector: Nickase (Nt.BspQI)-generated T-vector bearing a reddish-orange indicator gene
T-vectors are widely used for cloning the polymerase chain reaction (PCR) products. However, the low conversion efficiency of a plasmid into the linear T-vector usually results in non-recombinants. Here, we designed a new plasmid pNBQ-T to easily select the recombinant colonies harboring PCR product...
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| Publicado en: | Tissue Eng Regen Med |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Korean Tissue Engineering and Regenerative Medicine Society
2016
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6170991/ https://ncbi.nlm.nih.gov/pubmed/30603386 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13770-015-0118-z |
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