Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I

Crystals of site-specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 57.76, b = 90.67...

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Autori principali: Kachalova, Galina S., Rogulin, Eugeny A., Artyukh, Rimma I., Perevyazova, Tatyana A., Zheleznaya, Ludmila A., Matvienko, Nickolay I., Bartunik, Hans D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2005
Soggetti:
Crystallization Communications
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952280/
https://ncbi.nlm.nih.gov/pubmed/16511033
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910500309X
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