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Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast

CRISPR-Cas9 loss of function (LOF) and base editing screens are powerful tools in genetics and genomics. Yeast is one of the main models in these fields, but has only recently started to adopt this new toolkit for high throughput experiments. We developed a double selection strategy based on co-sele...

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Bibliografische gegevens
Gepubliceerd in:G3 (Bethesda)
Hoofdauteurs: Després, Philippe C, Dubé, Alexandre K, Nielly-Thibault, Lou, Yachie, Nozomu, Landry, Christian R
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Genetics Society of America 2018
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6169390/
https://ncbi.nlm.nih.gov/pubmed/30097473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.118.200461
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