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Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast

CRISPR-Cas9 loss of function (LOF) and base editing screens are powerful tools in genetics and genomics. Yeast is one of the main models in these fields, but has only recently started to adopt this new toolkit for high throughput experiments. We developed a double selection strategy based on co-sele...

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Publicat a:G3 (Bethesda)
Autors principals: Després, Philippe C, Dubé, Alexandre K, Nielly-Thibault, Lou, Yachie, Nozomu, Landry, Christian R
Format: Artigo
Idioma:Inglês
Publicat: Genetics Society of America 2018
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6169390/
https://ncbi.nlm.nih.gov/pubmed/30097473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.118.200461
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