Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

BACKGROUND: When developing CRISPR/Cas9 systems for crops, it is crucial to invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes, especially if the transformation system is difficult or time-consuming. Cotton is an important crop for the production of fiber, oil, and...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Plant Methods
Hauptverfasser: Long, Lu, Guo, Dan-Dan, Gao, Wei, Yang, Wen-Wen, Hou, Li-Pan, Ma, Xiao-Nan, Miao, Yu-Chen, Botella, Jose Ramon, Song, Chun-Peng
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2018
Schlagworte:
Methodology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6169012/
https://ncbi.nlm.nih.gov/pubmed/30305839
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-018-0353-0
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