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Reducing effects of particle adsorption to the air-water interface in cryoEM
Most protein particles prepared in vitreous ice for single particle cryo-electron microscopy are adsorbed to air-water or substrate-water interfaces, potentially causing particles to adopt preferred orientations. Using the Spotiton robot and nanowire grids, we can reduce some of the deleterious effe...
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| Veröffentlicht in: | Nat Methods |
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| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6168394/ https://ncbi.nlm.nih.gov/pubmed/30250056 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-018-0139-3 |
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