Routine single particle CryoEM sample and grid characterization by tomography

Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Noble, Alex J, Dandey, Venkata P, Wei, Hui, Brasch, Julia, Chase, Jillian, Acharya, Priyamvada, Tan, Yong Zi, Zhang, Zhening, Kim, Laura Y, Scapin, Giovanna, Rapp, Micah, Eng, Edward T, Rice, William J, Cheng, Anchi, Negro, Carl J, Shapiro, Lawrence, Kwong, Peter D, Jeruzalmi, David, des Georges, Amedee, Potter, Clinton S, Carragher, Bridget
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2018
Assuntos:
Structural Biology and Molecular Biophysics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5999397/
https://ncbi.nlm.nih.gov/pubmed/29809143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.34257
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