Routine single particle CryoEM sample and grid characterization by tomography

Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the...

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Veröffentlicht in:eLife
Hauptverfasser: Noble, Alex J, Dandey, Venkata P, Wei, Hui, Brasch, Julia, Chase, Jillian, Acharya, Priyamvada, Tan, Yong Zi, Zhang, Zhening, Kim, Laura Y, Scapin, Giovanna, Rapp, Micah, Eng, Edward T, Rice, William J, Cheng, Anchi, Negro, Carl J, Shapiro, Lawrence, Kwong, Peter D, Jeruzalmi, David, des Georges, Amedee, Potter, Clinton S, Carragher, Bridget
Format: Artigo
Sprache:Inglês
Veröffentlicht: eLife Sciences Publications, Ltd 2018
Schlagworte:
Structural Biology and Molecular Biophysics
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5999397/
https://ncbi.nlm.nih.gov/pubmed/29809143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.34257
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