Routine single particle CryoEM sample and grid characterization by tomography

Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the...

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Bibliografische gegevens
Gepubliceerd in:eLife
Hoofdauteurs: Noble, Alex J, Dandey, Venkata P, Wei, Hui, Brasch, Julia, Chase, Jillian, Acharya, Priyamvada, Tan, Yong Zi, Zhang, Zhening, Kim, Laura Y, Scapin, Giovanna, Rapp, Micah, Eng, Edward T, Rice, William J, Cheng, Anchi, Negro, Carl J, Shapiro, Lawrence, Kwong, Peter D, Jeruzalmi, David, des Georges, Amedee, Potter, Clinton S, Carragher, Bridget
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: eLife Sciences Publications, Ltd 2018
Onderwerpen:
Structural Biology and Molecular Biophysics
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5999397/
https://ncbi.nlm.nih.gov/pubmed/29809143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.34257
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