Routine single particle CryoEM sample and grid characterization by tomography

Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the...

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Опубликовано в: :eLife
Главные авторы: Noble, Alex J, Dandey, Venkata P, Wei, Hui, Brasch, Julia, Chase, Jillian, Acharya, Priyamvada, Tan, Yong Zi, Zhang, Zhening, Kim, Laura Y, Scapin, Giovanna, Rapp, Micah, Eng, Edward T, Rice, William J, Cheng, Anchi, Negro, Carl J, Shapiro, Lawrence, Kwong, Peter D, Jeruzalmi, David, des Georges, Amedee, Potter, Clinton S, Carragher, Bridget
Формат: Artigo
Язык:Inglês
Опубликовано: eLife Sciences Publications, Ltd 2018
Предметы:
Structural Biology and Molecular Biophysics
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5999397/
https://ncbi.nlm.nih.gov/pubmed/29809143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.34257
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