Routine single particle CryoEM sample and grid characterization by tomography

Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the...

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Sparad:
Bibliografiska uppgifter
I publikationen:eLife
Huvudupphovsmän: Noble, Alex J, Dandey, Venkata P, Wei, Hui, Brasch, Julia, Chase, Jillian, Acharya, Priyamvada, Tan, Yong Zi, Zhang, Zhening, Kim, Laura Y, Scapin, Giovanna, Rapp, Micah, Eng, Edward T, Rice, William J, Cheng, Anchi, Negro, Carl J, Shapiro, Lawrence, Kwong, Peter D, Jeruzalmi, David, des Georges, Amedee, Potter, Clinton S, Carragher, Bridget
Materialtyp: Artigo
Språk:Inglês
Publicerad: eLife Sciences Publications, Ltd 2018
Ämnen:
Structural Biology and Molecular Biophysics
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5999397/
https://ncbi.nlm.nih.gov/pubmed/29809143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.34257
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