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dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase

To develop structural modifications of dNTPs that are compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in charge and charge distribution throughout the fluorophore. These dUTP derivatives and commercial Cy3− and...

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Detaylı Bibliyografya
Yayımlandı:Nucleic Acids Res
Asıl Yazarlar: Zasedateleva, Olga A, Vasiliskov, Vadim A, Surzhikov, Sergey A, Kuznetsova, Viktoriya E, Shershov, Valeriy E, Guseinov, Timur O, Smirnov, Igor P, Yurasov, Roman A, Spitsyn, Maksim A, Chudinov, Alexander V
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 2018
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6158613/
https://ncbi.nlm.nih.gov/pubmed/29648660
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky247
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