dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase

To develop structural modifications of dNTPs that are compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in charge and charge distribution throughout the fluorophore. These dUTP derivatives and commercial Cy3− and...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Zasedateleva, Olga A, Vasiliskov, Vadim A, Surzhikov, Sergey A, Kuznetsova, Viktoriya E, Shershov, Valeriy E, Guseinov, Timur O, Smirnov, Igor P, Yurasov, Roman A, Spitsyn, Maksim A, Chudinov, Alexander V
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6158613/
https://ncbi.nlm.nih.gov/pubmed/29648660
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky247
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