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TRUmiCount: correctly counting absolute numbers of molecules using unique molecular identifiers

MOTIVATION: Counting molecules using next-generation sequencing (NGS) suffers from PCR amplification bias, which reduces the accuracy of many quantitative NGS-based experimental methods such as RNA-Seq. This is true even if molecules are made distinguishable using unique molecular identifiers (UMIs)...

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Detalhes bibliográficos
Publicado no:Bioinformatics
Main Authors: Pflug, Florian G, von Haeseler, Arndt
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6157883/
https://ncbi.nlm.nih.gov/pubmed/29672674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/bioinformatics/bty283
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