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Gaussia princeps luciferase: a bioluminescent substrate for oxidative protein folding

Gaussia princeps luciferase (GLuc) generates an intense burst of blue light when exposed to coelenterazine in the absence of ATP. Here we show that this 5‐disulfide containing enzyme can be used as a facile and convenient substrate for studies of oxidative protein folding. Reduced GLuc (rGLuc), with...

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Bibliografiske detaljer
Udgivet i:Protein Sci
Main Authors: Yu, Tiantian, Laird, Joanna R., Prescher, Jennifer A., Thorpe, Colin
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley and Sons Inc. 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6153383/
https://ncbi.nlm.nih.gov/pubmed/29696739
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3433
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