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Gaussia princeps Luciferase as a Reporter for Transcriptional Activity, Protein Secretion, and Protein-Protein Interactions in Salmonella enterica Serovar Typhimurium

Gaussia princeps luciferase (Gluc) is widely used as a reporter in eukaryotes, but data about its applicability in bacteria are very limited. Here we show that a codon-optimized Gluc gene can be efficiently expressed in Salmonella enterica serovar Typhimurium. To test different Gluc variants as tran...

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Main Authors: Wille, Thorsten, Blank, Kathrin, Schmidt, Christiane, Vogt, Vivien, Gerlach, Roman G.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3255616/
https://ncbi.nlm.nih.gov/pubmed/22020521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.06670-11
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