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Optimization of Enzyme-Substrate Pairing for Bioluminescence Imaging of Gene Transfer Using Renilla and Gaussia Luciferases

BACKGROUND: Bioluminescence imaging (BLI) permits the noninvasive quantitation and localization of transduction and expression by gene transfer vectors. The tendency of tissue to attenuate light in the optical region, however, limits the sensitivity of BLI. Improvements in light output from biolumin...

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Autors principals: Kimura, Takahiro, Hiraoka, Kei, Kasahara, Noriyuki, Logg, Christopher R.
Format: Artigo
Idioma:Inglês
Publicat: 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2989363/
https://ncbi.nlm.nih.gov/pubmed/20527045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jgm.1463
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