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An NMR strategy to detect conformational differences in a protein complexed with highly analogous inhibitors in solution

This manuscript presents an NMR strategy to investigate conformational differences in protein-inhibitor complexes, when the inhibitors tightly bind to a protein at sub-nanomolar dissociation constants and are highly analogous to each other. Using HIV-1 protease (PR), we previously evaluated amide ch...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Persons, John D., Khan, Shahid N., Ishima, Rieko
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6133750/
https://ncbi.nlm.nih.gov/pubmed/29656080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.04.005
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