Engineering of 3-ketosteroid-∆(1)-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates

BACKGROUND: Biosynthesis of steroidal drugs is of great benefit in pharmaceutical manufacturing as the process involves efficient enzymatic catalysis at ambient temperature and atmospheric pressure compared to chemical synthesis. 3-ketosteroid-∆(1)-dehydrogenase from Arthrobacter simplex (KsdD3) cat...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Mao, Shuhong, Wang, Jian-Wen, Liu, Fufeng, Zhu, Zhangliang, Gao, Dengke, Guo, Qianqian, Xu, Panpan, Ma, Zheng, Hou, Yali, Cheng, Xiaotao, Sun, Dengyue, Lu, Fuping, Qin, Hui-Min
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6130075/
https://ncbi.nlm.nih.gov/pubmed/30200975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-018-0981-0
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