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Rational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates
Cholesterol oxidase catalyzes the oxidation and isomerization of the cholestane substrates leading to the addition of a hydroxyl group at the C3 position. Rational engineering of the cholesterol oxidase from Pimelobacter simplex (PsChO) was performed. Mutagenesis of V64 and F70 improved the catalyti...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5703901/ https://ncbi.nlm.nih.gov/pubmed/29180806 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-16768-6 |
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