Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

Base editors enable targeted single-nucleotide conversion in genomic DNA. Here we show that expression levels are a bottleneck in base editing efficiency. We optimize cytidine (BE4) and adenine (ABE7.10) base editors by modification of nuclear localization signals and codon usage, and ancestral reco...

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Bibliografski detalji
Izdano u:Nat Biotechnol
Glavni autori: Koblan, Luke W., Doman, Jordan L., Wilson, Christopher, Levy, Jonathan M., Tay, Tristan, Newby, Greg A., Maianti, Juan Pablo, Raguram, Aditya, Liu, David R.
Format: Artigo
Jezik:Inglês
Izdano: 2018
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Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6126947/
https://ncbi.nlm.nih.gov/pubmed/29813047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4172
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