Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of <1000 mammalian cells

Multidimensional peptide separations can greatly increase the depth of coverage in proteome profiling. However, a major challenge for multidimensional separations is the requirement of large biological samples, often containing milligram amounts of protein. We have developed nanowell-mediated two-di...

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Detalhes bibliográficos
Publicado no:Chem Sci
Main Authors: Dou, Maowei, Zhu, Ying, Liyu, Andrey, Liang, Yiran, Chen, Jing, Piehowski, Paul D., Xu, Kerui, Zhao, Rui, Moore, Ronald J., Atkinson, Mark A., Mathews, Clayton E., Qian, Wei-Jun, Kelly, Ryan T.
Formato: Artigo
Idioma:Inglês
Publicado em: Royal Society of Chemistry 2018
Assuntos:
Chemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6124911/
https://ncbi.nlm.nih.gov/pubmed/30210768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c8sc02680g
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