Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples

Two-dimensional reversed-phase capillary liquid chromatography (2D RPLC) separations have enabled comprehensive proteome profiling of biological systems. However, milligram sample quantities of proteins are typically required due to significant losses during offline fractionation. Such a large sampl...

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Publicado en:Anal Chem
Main Authors: Dou, Maowei, Tsai, Chia-Feng, Piehowski, Paul D., Wang, Yang, Fillmore, Thomas L., Zhao, Rui, Moore, Ronald J., Zhang, Pengfei, Qian, Wei-Jun, Smith, Richard D., Liu, Tao, Kelly, Ryan T., Shi, Tujin, Zhu, Ying
Formato: Artigo
Idioma:Inglês
Publicado: 2019
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6741344/
https://ncbi.nlm.nih.gov/pubmed/31241912
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.9b01248
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