Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell

We report on the combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS), and the latest-generation Orbitrap Eclipse Tribrid mass spectrometer for greatly improved single-cell proteome profiling. FAIMS effectively filtered out sin...

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Detalhes bibliográficos
Publicado no:Chem Sci
Main Authors: Cong, Yongzheng, Motamedchaboki, Khatereh, Misal, Santosh A., Liang, Yiran, Guise, Amanda J., Truong, Thy, Huguet, Romain, Plowey, Edward D., Zhu, Ying, Lopez-Ferrer, Daniel, Kelly, Ryan T.
Formato: Artigo
Idioma:Inglês
Publicado em: The Royal Society of Chemistry 2020
Assuntos:
Chemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8178986/
https://ncbi.nlm.nih.gov/pubmed/34163866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0sc03636f
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