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Multiplexed deactivated CRISPR-Cas9 gene expression perturbations deter bacterial adaptation by inducing negative epistasis

The ever-increasing threat of multi-drug resistant bacteria, a shrinking antibiotic pipeline, and the innate ability of microorganisms to adapt necessitates long-term strategies to slow the evolution of antibiotic resistance. Here we develop an approach, dubbed Controlled Hindrance of Adaptation of...

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Detalhes bibliográficos
Publicado no:Commun Biol
Main Authors: Otoupal, Peter B., Cordell, William T., Bachu, Vismaya, Sitton, Madeleine J., Chatterjee, Anushree
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6123780/
https://ncbi.nlm.nih.gov/pubmed/30272008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-018-0135-2
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