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Template-primer binding affinity and RNase H cleavage specificity contribute to the strand transfer efficiency of HIV-1 reverse transcriptase

During reverse transcription of the HIV-1 genome, two strand-transfer events occur. Both events rely on the RNase H cleavage activity of reverse transcriptases (RTs) and template homology. Using a panel of mutants of HIV-1(BH10) (group M/subtype B) and HIV-1(ESP49) (group O) RTs and in vitro assays,...

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Vydáno v:J Biol Chem
Hlavní autoři: Luczkowiak, Joanna, Matamoros, Tania, Menéndez-Arias, Luis
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6120193/
https://ncbi.nlm.nih.gov/pubmed/29991591
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA118.004324
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