Discovery and engineering of enhanced SUMO protease enzymes

Small ubiquitin-like modifier (SUMO) is commonly used as a protein fusion domain to facilitate expression and purification of recombinant proteins, and a SUMO-specific protease is then used to remove SUMO from these proteins. Although this protease is highly specific, its limited solubility and stab...

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Dettagli Bibliografici
Pubblicato in:J Biol Chem
Autori principali: Lau, Yue-Ting K., Baytshtok, Vladimir, Howard, Tessa A., Fiala, Brooke M., Johnson, JayLee M., Carter, Lauren P., Baker, David, Lima, Christopher D., Bahl, Christopher D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Biochemistry and Molecular Biology 2018
Soggetti:
Enzymology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6109922/
https://ncbi.nlm.nih.gov/pubmed/29976752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA118.004146
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