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Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

Huntington's Disease (HD) is an inherited fatal neurodegenerative disease caused by a CAG expansion (≥36) in the first exon of the HD gene, resulting in the expression of the Huntingtin protein (Htt) or N-terminal fragments thereof with an expanded polyglutamine (polyQ) stretch. The exon1 of th...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Reif, Andreas, Chiki, Anass, Ricci, Jonathan, Lashuel, Hilal A.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6102005/
https://ncbi.nlm.nih.gov/pubmed/30010666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57506
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