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An Intein-based Strategy for the Production of Tag-free Huntingtin Exon 1 Proteins Enables New Insights into the Polyglutamine Dependence of Httex1 Aggregation and Fibril Formation

The first exon of the Huntingtin protein (Httex1) is one of the most actively studied Htt fragments because its overexpression in R6/2 transgenic mice has been shown to recapitulate several key features of Huntington disease. However, the majority of biophysical studies of Httex1 are based on assess...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Vieweg, Sophie, Ansaloni, Annalisa, Wang, Zhe-Ming, Warner, John B., Lashuel, Hilal A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4933259/
https://ncbi.nlm.nih.gov/pubmed/27002149
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M116.713982
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