Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

CRISPR/Cas9 technology is accelerating genome engineering in many cell types, but so far, gene delivery and stable gene modification have been challenging in primary NK cells. For example, transgene delivery using lentiviral or retroviral transduction resulted in a limited yield of genetically-engin...

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Enregistré dans:
Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Naeimi Kararoudi, Meisam, Dolatshad, Hamid, Trikha, Prashant, Hussain, Syed-Rehan A., Elmas, Ezgi, Foltz, Jennifer A., Moseman, Jena E., Thakkar, Aarohi, Nakkula, Robin J., Lamb, Margaret, Chakravarti, Nitin, McLaughlin, K. John, Lee, Dean A.
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2018
Sujets:
Immunology and Infection
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101749/
https://ncbi.nlm.nih.gov/pubmed/29985369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58237
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