Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

CRISPR/Cas9 technology is accelerating genome engineering in many cell types, but so far, gene delivery and stable gene modification have been challenging in primary NK cells. For example, transgene delivery using lentiviral or retroviral transduction resulted in a limited yield of genetically-engin...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Naeimi Kararoudi, Meisam, Dolatshad, Hamid, Trikha, Prashant, Hussain, Syed-Rehan A., Elmas, Ezgi, Foltz, Jennifer A., Moseman, Jena E., Thakkar, Aarohi, Nakkula, Robin J., Lamb, Margaret, Chakravarti, Nitin, McLaughlin, K. John, Lee, Dean A.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Immunology and Infection
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101749/
https://ncbi.nlm.nih.gov/pubmed/29985369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58237
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