Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

The main aim of this protocol is to describe the development and validation of an interferon (IFN)-α single molecule array digital Enzyme-Linked ImmunoSorbent Assay (ELISA) assay. This system enables the quantification of human IFN-α protein with unprecedented sensitivity, and with no cross-reactivi...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Llibre, Alba, Bondet, Vincent, Rodero, Mathieu P., Hunt, David, Crow, Yanick J., Duffy, Darragh
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Immunology and Infection
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101729/
https://ncbi.nlm.nih.gov/pubmed/29985347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57421
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