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Single Molecule Enzyme-Linked Immunosorbent Assays: Theoretical Considerations

We have developed a highly sensitive immunoassay—called digital ELISA—that is based on the detection of single enzyme-linked immunocomplexes on beads that are sealed in arrays of femtoliter wells. Digital ELISA was designed to be highly efficient in the capturing of target proteins, labeling of thes...

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Detalhes bibliográficos
Main Authors: Chang, Lei, Rissin, David M., Fournier, David R., Piech, Tomasz, Patel, Purvish P., Wilson, David H., Duffy, David C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3327511/
https://ncbi.nlm.nih.gov/pubmed/22370429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2012.02.011
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