Improved sgRNA design in bacteria via genome-wide activity profiling

CRISPR/Cas9 is a promising tool in prokaryotic genome engineering, but its success is limited by the widely varying on-target activity of single guide RNAs (sgRNAs). Based on the association of CRISPR/Cas9-induced DNA cleavage with cellular lethality, we systematically profiled sgRNA activity by co-...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Guo, Jiahui, Wang, Tianmin, Guan, Changge, Liu, Bing, Luo, Cheng, Xie, Zhen, Zhang, Chong, Xing, Xin-Hui
Format: Artigo
Language:Inglês
Published: Oxford University Press 2018
Subjects:
Data Resources and Analyses
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101607/
https://ncbi.nlm.nih.gov/pubmed/29982721
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky572
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